Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.4.1.9 extracted from

  • Wang, Y.; Hou, Y.; Wang, Y.; Zheng, L.; Xu, X.; Pan, K.; Li, R.; Wang, Q.
    A novel cold-adapted leucine dehydrogenase from antarctic sea-ice bacterium Pseudoalteromonas sp. ANT178 (2018), Mar. Drugs, 16, 359 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.4.1.9

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pseudoalteromonas sp. ANT 178

Crystallization (Commentary)

Crystallization (Comment) Organism
homology modeling of structure. Enzyme exhibits several cold-adapted features Pseudoalteromonas sp. ANT 178

Inhibitors

Inhibitors Comment Organism Structure
Cd2+ 1 mM, 8% residual activity Pseudoalteromonas sp. ANT 178
Cr2+ 1 mM, 13% residual activity Pseudoalteromonas sp. ANT 178
Cu2+ 1 mM, 61% residual activity Pseudoalteromonas sp. ANT 178
EDTA 1 mM, 92% residual activity Pseudoalteromonas sp. ANT 178
Fe2+ 1 mM, 75% residual activity Pseudoalteromonas sp. ANT 178
Hg2+ 1 mM, 29% residual activity Pseudoalteromonas sp. ANT 178
Pb2+ 1 mM, no residual activity Pseudoalteromonas sp. ANT 178
Thiourea 1 mM, 52% residual activity Pseudoalteromonas sp. ANT 178
Zn2+ 1 mM, 80% residual activity Pseudoalteromonas sp. ANT 178

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.33
-
 L-leucine pH 9, 30°C Pseudoalteromonas sp. ANT 178
0.48
-
 L-tyrosine pH 9, 30°C Pseudoalteromonas sp. ANT 178
0.64
-
 L-proline pH 9, 30°C Pseudoalteromonas sp. ANT 178
0.75
-
 DL-methionine pH 9, 30°C Pseudoalteromonas sp. ANT 178
0.84
-
 L-arginine pH 9, 30°C Pseudoalteromonas sp. ANT 178

Metals/Ions

Metals/Ions Comment Organism Structure
NaCl 2 M NaCl, 140% of initial activity Pseudoalteromonas sp. ANT 178

Organism

Organism UniProt Comment Textmining
Pseudoalteromonas sp. ANT 178 A0A385GJJ6
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
275
-
 pH 9, 30°C, substrate L-leucine Pseudoalteromonas sp. ANT 178

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DL-methionine + H2O + NAD+
-
 Pseudoalteromonas sp. ANT 178 3-methylthio-2-oxobutanoate + NH3 + NADH + H+
-
 ?
L-arginine + H2O + NAD+
-
 Pseudoalteromonas sp. ANT 178 5-[(diaminomethyl)amino]-2-oxopentanoic acid + NH3 + NADH + H+
-
 ?
L-leucine + H2O + NAD+
-
 Pseudoalteromonas sp. ANT 178 4-methyl-2-oxopentanoate + NH3 + NADH + H+
-
 ?
L-proline + H2O + NAD+
-
 Pseudoalteromonas sp. ANT 178 ? + NH3 + NADH + H+
-
 ?
L-tyrosine + H2O + NAD+
-
 Pseudoalteromonas sp. ANT 178 ? + NH3 + NADH + H+
-
 ?

Subunits

Subunits Comment Organism
? x * 42600, calculated from sequence, x * 44000, SDS-PAGE, recombinant protein Pseudoalteromonas sp. ANT 178

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Pseudoalteromonas sp. ANT 178

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
 120 min, 85% residual activity Pseudoalteromonas sp. ANT 178

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
14.1
-
 L-arginine pH 9, 30°C Pseudoalteromonas sp. ANT 178
16.6
-
 DL-methionine pH 9, 30°C Pseudoalteromonas sp. ANT 178
20.8
-
 L-proline pH 9, 30°C Pseudoalteromonas sp. ANT 178
26.4
-
 L-tyrosine pH 9, 30°C Pseudoalteromonas sp. ANT 178
30.13
-
 L-leucine pH 9, 30°C Pseudoalteromonas sp. ANT 178

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
 Pseudoalteromonas sp. ANT 178

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
16.8
-
 L-arginine pH 9, 30°C Pseudoalteromonas sp. ANT 178
22.1
-
 DL-methionine pH 9, 30°C Pseudoalteromonas sp. ANT 178
32.5
-
 L-proline pH 9, 30°C Pseudoalteromonas sp. ANT 178
55
-
 L-tyrosine pH 9, 30°C Pseudoalteromonas sp. ANT 178
91.3
-
 L-leucine pH 9, 30°C Pseudoalteromonas sp. ANT 178